A loxP site was inserted upstream of exon 2 and an frt flanked neo cassette with a 5' loxP site was inserted upstream of exon 2. The selection cassette was deleted by Flpe recombinase. (J:155885)
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A loxP site was inserted upstream of exon 2 and an frt flanked neo cassette with a 5' loxP site was inserted upstream of exon 2. The selection cassette was deleted by Flpe recombinase. (J:155885)