An frt and loxP flanked neo cassette was inserted upstream of exon 2 and an additional loxP was inserted downstream of exon 3. Flp mediated recombination removed the neo cassette leaving exons 2 and 3 floxed. (J:155398)
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An frt and loxP flanked neo cassette was inserted upstream of exon 2 and an additional loxP was inserted downstream of exon 3. Flp mediated recombination removed the neo cassette leaving exons 2 and 3 floxed. (J:155398)