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Briefly, 5.9 kb of upstream and 2.2 kb of downstream Tbx2 129/SvJ genomic fragments were amplified by PCR and ligated to an Frt-flanked Cre-polyA-PGK-neo cassette replacing the first five codons of the Tbx2-coding region in exon 1. A diphteria toxin negative-selection cassette was placed at the 5' end of the targeting construct. Homologous recombination was performed in E14IB10 ES cells. The PGK-neo cassette in Tbx2CreNEO animals was removed subsequently by crossing with FlpE mice. Tbx2 expression is undetectable in homozygous embryos which show a similar hindlimb phenotype to that observed in Tbx2-deficient (Tbx2tm1Pa) embryos. (J:155719)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
