A loxP site was inserted upstream of exon 1 and an frt flanked neo cassette with a 3' loxP site was inserted downstream of exon 3. Flp mediated recombination removed the neo cassette leaving exons 1 through 3 floxed. (J:155427)
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A loxP site was inserted upstream of exon 1 and an frt flanked neo cassette with a 3' loxP site was inserted downstream of exon 3. Flp mediated recombination removed the neo cassette leaving exons 1 through 3 floxed. (J:155427)