A floxed neo cassette was inserted 30 bp downstream of the translation initiation site. Cre mediated recombination removed the neo cassette leaving a single loxP site. The absence of COX activity was confirmed in E8.5 embryos. (J:155116)
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A floxed neo cassette was inserted 30 bp downstream of the translation initiation site. Cre mediated recombination removed the neo cassette leaving a single loxP site. The absence of COX activity was confirmed in E8.5 embryos. (J:155116)