Log In|Sign Up
EN
LoxP sites were inserted 5' and 3' of the keratin type II gene cluster, encompassing 0.68 Mb, 26 type II genes and 1 keratin type I gene (Krt18). Cre mediated recombination deleted the entire cluster leaving a single loxP site flanked by 3' and 5' hprt mini-genes and the the tyrosinase (Tyr) and agouti (A) coat color genes. The absence of the transcripts and gene products was confirmed by RT-PCR and immunofluorescence microscopy in homozygous embryos. Due to the absence of all type II keratins, all the type I keratins were sensitive to proteolysis, so homozygous mice lack all 54 keratin proteins. ES cells carrying Krt1ctm1Tmm and Igs11tm1Tmm and were used to create this deletion. (J:153804)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
Show/Hide columns
| Phenotypes |
|---|
References Literature
Title
PMID
Journal
Year
IF
