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A DNA fragment encoding cre/ERT2 (cre recombinase fused to the human estrogen receptor with a mutated ligand-binding domain) was inserted downstream of a 6.3-kb fragment of the human CD19 promoter. This construct was inserted into the LC-1 BAC via Red-mediated homologous recombination in E. coli. The recombinant BAC was injected into the pronuclei of fertilized C57BL/6 oocytes. Two founders were generated and one line designated LC-1-hCD19-CreERT2 provided functional cre-mediated recombination. PCR analysis showed that animals carried a single copy of the transgene. (J:154796)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
