Ldah^{tm1(KOMP)Vlcg}

The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 18006bp between positions 8270605-8288610 of Chromosome 12 (Build GRCm39). Sequencing confirmed replacement of a region from exon 2, downstream of the start codon, to base 169 of exon 3, including the predicted catalytic Gx-SxG motif, with a lacZ reporter and a loxP-flanked neomycin cassette. Western blot analysis using an antibody binding a C-terminal antigen revealed no detectable protein in the liver, BAT and WAT, adrenal gland, prostate, brain, spleen, kidney, testis, and bone marrow-derived macrophages of homozygous mutant mice. Mass spectrometry analysis confirmed the absence of encoded peptides in WAT and liver lysates. (J:136110, J:237921)