The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 9754bp between positions 22288491-22298244 of Chromosome 6 (Build GRCm39). Nucleotides between position 266 and 10025 of the cDNA are replaced by the ZEN Ub1 cassette. This leads to a complete lack of Wnt16 mRNA transcription of the targeted locus. (J:136110, J:227816)