The targeting construct contained a cassette encoding an IRES-cre sequence followed by an frt-flanked neo selection marker. The construct was targeted to a position 3 nucleotides downstream of the Olfr157 coding sequence. After linearization the construct was injected into R1 ES cells. Clones were injected into C57BL/6J blastocysts and germline transmission was obtained. These mice were crossed to mice expressing flp recombinase to remove the frt-neo-frt sequence to ascertain if its presence induced an artfacts in the observed expression of the allele. (J:154249)
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基础信息

模型ID
品系来源
等位基因类型
突变
遗传方式
相关基因
相关疾病
参考文献
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Targeted
Insertion
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1
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1

表型特征

标签摘要:
hm: 纯合子
ht: 杂合子
cn: 条件基因型
cx: 复合型:涉及多基因组
tg: 转基因
ot: 其他:半合子、不确定...
(F): 雌性
(M): 雄性
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N: 正常表型
(#): 上标括号内为相关疾病数量
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