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The transgenic construct has the Pgk1 promoter and the EGFP gene separated by a floxed STOP cassette containing a puromycin resistance selection marker, two poly A sites and a trasncriptional pause cassette (synthetic pA site and a transcriptional pause site). The linearized construct was electroporated into E14TG2a subclone IV ES cells. Positive clones were injected into day 3.5 C57BL/6 blastocysts and chimeras were bred to C57BL/6 mice to establish the transgenic line. (J:135901)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
