A conditional-ready gene trap cassette with FRT, F3, loxP, and Lox5171 sites upstream of a beta-geo reporter followed downstream by loxP, lox5171, FRT, and F3 sites in inverse orientation, was inserted into exon 1. This insertion is in the original gene-trapped configuration so that transcription is stopped at the beta-geo reporter, yielding a null allele, which can be excised into a functional allele through Flp- or Cre-mediated excision. (J:236978)
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129P2/OlaHsd
Gene trapped
Insertion of gene trap vector
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1
6
1

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标签摘要:
hm: 纯合子
ht: 杂合子
cn: 条件基因型
cx: 复合型:涉及多基因组
tg: 转基因
ot: 其他:半合子、不确定...
(F): 雌性
(M): 雄性
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N: 正常表型
(#): 上标括号内为相关疾病数量
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