A loxP site was inserted upstream of exon 1 and an frt flanked neo cassette with a 5' loxP site was inserted downstream of exon 1. Germ line, flp mediated recombination removes the neo cassette leaving exon 1 floxed. (J:154336)
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A loxP site was inserted upstream of exon 1 and an frt flanked neo cassette with a 5' loxP site was inserted downstream of exon 1. Germ line, flp mediated recombination removes the neo cassette leaving exon 1 floxed. (J:154336)