A loxP site was inserted upstream of exon 3, and an frt flanked neo cassette with a 3' loxP site was inserted downstream of exon 4. Germ line, flp mediated recombination removed the neo cassette leaving exons 3 and 4 floxed. (J:153774)
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A loxP site was inserted upstream of exon 3, and an frt flanked neo cassette with a 3' loxP site was inserted downstream of exon 4. Germ line, flp mediated recombination removed the neo cassette leaving exons 3 and 4 floxed. (J:153774)