Tg(TetO-MYC*)4Gev

This transgene permits conditional inhibition of MYC via expression, driven by the cytomegalovirus (CMV) early promoter and regulated by the tetracycline responsive element (TRE or TetO), of a dominant interfering mutant MYC bHLHZip dimerization domain. The expressed protein is a human MYC-derived basic helix-loop-helix zipper (bHLHZip) domain in which replacement of four charged amino acids (Glu57Thr, Glu64Ile, Arg70Glu, Arg71Asn) permits it to form homodimers, as well as heterodimers with endogenous MYC that interfere with the formation of MYC/MAX dimers and suppress binding to E-box elements. In doubly transgenic mice also expressing the tetracycline transactivating or reverse transactivating element (tTA or rtTA), expression of the MYC inhibitor can be regulated by doxycycline administration or removal. (J:141029, J:153799)