A loxP site was inserted upstream of exon 2 and an frt flaned neo cassette with a 3' loxP site was inserted downstream of exon 5. Germ line, flp mediated recombination removed the neo cassette leaving exons 2 through 5 floxed. (J:153555)
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A loxP site was inserted upstream of exon 2 and an frt flaned neo cassette with a 3' loxP site was inserted downstream of exon 5. Germ line, flp mediated recombination removed the neo cassette leaving exons 2 through 5 floxed. (J:153555)