A loxP site was inserted upstream of exon 3 and an frt flanked neo cassette with a 5' loxP site was inserted downstream of exon 4. The neo cassette was removed by germ line, flp mediated recombination. (J:153219)
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A loxP site was inserted upstream of exon 3 and an frt flanked neo cassette with a 5' loxP site was inserted downstream of exon 4. The neo cassette was removed by germ line, flp mediated recombination. (J:153219)