Usp22^{tm1a(KOMP)Wtsi}

The L1L2_Bact_P cassette was inserted at position 61058865 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 61059653. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml Special note about mutant cell line EPD0119_2_D11 only: The mutation generated in this ES cell line results in a hypomorphic allele. qRT-PCR analysis revealed a minimal level (~3%) of residual expression of correctly spliced mRNA in brain and small intestine tissues isolated from adult homozygous mutant mice. Western blot analysis confirmed a significant but incomplete reduction of protein levels in both tissues (J:233975). Other cell lines associated with this allele have not been tested. (J:148605, J:173534)