A loxP site was inserted upstream of exon 10 and a floxed neo cassette was inserted downstream of exon 12 via homologous recombination. The neo cassette was then removed by Cre mediated recombination leaving exons 10 - 12 floxed. (J:115188)
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A loxP site was inserted upstream of exon 10 and a floxed neo cassette was inserted downstream of exon 12 via homologous recombination. The neo cassette was then removed by Cre mediated recombination leaving exons 10 - 12 floxed. (J:115188)