A floxed neo cassette was inserted upstream of exon 2 and a single loxP site was inserted downstream of exon 2 via homologous recombination. The neo cassette was then removed by Cre mediated recombination, leaving exon 2 floxed. (J:152912)
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A floxed neo cassette was inserted upstream of exon 2 and a single loxP site was inserted downstream of exon 2 via homologous recombination. The neo cassette was then removed by Cre mediated recombination, leaving exon 2 floxed. (J:152912)