Mnx1^tm1Slp

Exon 3 of the targeted gene was flanked by loxP sites and a neomycin cassette flanked by FRT sites was introduced to intron 2. The targeting vector was electroporated into 129S1/Sv Oca2+ Tyr+ Kitl +-derived W9.5 embryonic stem (ES) cells. Chimeric animals were crossed to C57BL/6 and then to CB6F1 background FLP mice to remove the neomycin cassette before the strain was bred to homozygosity. (J:152526)