An frt flanked neo cassette was inserted upstream of exon 2 and loxP site was inserted flanking exons 2 through 4. Flp mediated recombination removed the neo cassette leaving exons 2 through 4 floxed. (J:152259)
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An frt flanked neo cassette was inserted upstream of exon 2 and loxP site was inserted flanking exons 2 through 4. Flp mediated recombination removed the neo cassette leaving exons 2 through 4 floxed. (J:152259)