Prdm1^tm2.1Nutt

A loxP site was inserted upstream of exon 6 with an additional loxP site and a frt-flanked neomycin/EGFP cassette inserted downstream of exon 6. The neomycin/EGFP cassette was subsequently removed by crossing founder mice with a flpe transgenic mice, leaving exon 6 floxed. Removal of exon 6 by cre-mediated recombination will leave exon 7 out-of-frame and create a null allele. (J:151858)