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A loxP site was inserted 42 bp upstream of the lone coding exon and a Frt-flanked neomycin cassette, another loxP site, and a myc-tagged luciferase coding sequence were inserted downstream of the exon in the BstAPI restriction site. After targeting in C57BL/6 ES cells, the neomycin cassette was removed by crossing founder mice with FLP-deleter mice. A poly-A sequence upstream of the luciferase cassette prevents transcription of the reporter until the endogenous exon is floxed. (J:151854)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
