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Exon 6 of Gucy1a3 gene which codes for a conserved portion of the catalytic domain was flanked by a loxP and a loxP-neo-loxP cassette. The exon 6 and neo cassette was removed in ES cells by transient cre expression leaving a single loxP in place. Northern blot and RT-PCR confirmed excision of exon 6 in the transcript; however, immunoblot revealed a smaller mutant 59-kDa protein in homozygous mice. Mutant protein was determined to be inactive, thus it is functional null allele. (J:151161)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
