A loxP site was inserted upstream of exon 2 and an frt flanked neo cassette with a 3' loxP site was inserted downstream of exon 2. The neo cassette was removed by flp mediated recombination leaving exon 2 floxed. (J:150962)
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A loxP site was inserted upstream of exon 2 and an frt flanked neo cassette with a 3' loxP site was inserted downstream of exon 2. The neo cassette was removed by flp mediated recombination leaving exon 2 floxed. (J:150962)