A loxP site was inserted upstream of exon 13, which encodes the transmembrane domain, and an frt flanked neo cassette and second loxP site were inserted downstream of exon 13. Flp mediated recombination then removed the neo cassette. (J:150952)
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A loxP site was inserted upstream of exon 13, which encodes the transmembrane domain, and an frt flanked neo cassette and second loxP site were inserted downstream of exon 13. Flp mediated recombination then removed the neo cassette. (J:150952)