Tdp2^{Gt(RRS512)Byg}

The trapping vector pGT0lxf was inserted into intron 5. Quantitative PCR and FISH analysis indicated that more than 40 copies of the vector had integrated in a single site. No beta-galactosidase staining was detected during embryogenesis; however, lacZ mRNA expression is detected. RT-PCR analysis detected the presence of wild-type mRNA in homozygous trapped mice. Further analysis revealed a partial duplication of the wild-type gene including exons 1 - 5 that appeared to be inserted after the tandem vector insertion.