A gene trap vector (pGT0Lxf) composed of a splice acceptor site (SA), a beta-galactosidase-neomycin fusion protein (b-geo) and a polyA tail (pA) was inserted into intron 2. Quantification of protein levels showed that the knockout is incomplete leading to an 84% reduction in protein levels. (J:221848)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129P2/OlaHsd
Gene trapped
Insertion of gene trap vector
--
1
5
5

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
Journal
Year
IF
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