Trapped gene association determined by genome coordinate overlap and strand. Sequencing 5'-rapid amplification of the cDNA ends indicates that the gene trap vector pGT1dTMpfs inserted between exon 1 and exon 2. Western blot analysis on forebrain extracts confirmed the absence of protein expression. qPCR and Western blots confirmed absence of mRNA and protein expression in various tissues. (J:179214, J:214116)
Basic Information
Insertion of gene trap vector
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count