A gene trap cassette inserted in intron 1 contained a strong splice acceptor from En2 fused to the coding sequence for beta-galactosidase. Western blot analysis confirmed the absence of protein expression in homozygous mutant mice. (J:271138)
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A gene trap cassette inserted in intron 1 contained a strong splice acceptor from En2 fused to the coding sequence for beta-galactosidase. Western blot analysis confirmed the absence of protein expression in homozygous mutant mice. (J:271138)