Suco^{Gt(KST050)Byg}

The gene trap vector inserted at bp 1624 in intron 11. RT-PCR and northern blot analysis confirmed the absence of full length transcript in homozygous embryos. Immunoblot analysis detected negligible amounts of protein in homozygous whole embryo lysates. Beta-galactosidase activity was detected in all skeletal elements of newborn heterozygotes. (J:159823)