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A lacZ reporter poly A gene cassette with a splice acceptor was inserted into intron 1 of the gene before the ATG start codon in exon 2. Transcription at the locus should result in the splicing of exon 1 to the gene trap vector and eliminate the production of the endogenous transcript. RT-PCR analysis of total RNA from E9.5 embryos confirmed the absence of detectable mRNA in homozygous mutant samples. X-gal staining revealed that lacZ activity was present in ciliated tissues during mouse development including the node, the floor plate and the dorsal neural tube. (J:227136)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
