Gene trap cassette pGT01 was inserted into exon 2, upstream of the ATG translation start site. The single insertion site was confirmed by Southern blot and sequencing analyses. Ablation of mRNA and protein expression was confirmed by quantitative RT-PCR and Western blot. (J:250112)
Basic Information
Insertion of gene trap vector
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count