Gene trap vector pGT01xr, containing a splice acceptor and a lacZ/neomycin phosphotransferase (betageo) fusion gene, was inserted immediately after the sequence 5'-GCACTCCTCTGTCTGCCTTG-3' in intron 4. Western blot analysis confirmed the absence of protein expression in mouse embryonic fibroblasts (MEFs). (J:230736)
Basic Information
Insertion of gene trap vector
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count