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A BAC clone of >160kb from a CJ7/129Sv Mouse BAC library (Invitrogen, formerly Genome Systems Inc.) containing the two exons of Adora2a, flanked by at least 70 kb of Adora2a 5' upstream sequences. This A2AR BAC was modified by homologous recombination in bacteria to replace the sequence of exon 1 (after the ATG transcription start site) with the cre recombinase coding sequence including 3 STOP codons. The purified BAC construct was injected into fertilized B6SJLF1 zygotes. Two founder lines, 2M and 9F, wer established. Mice were crossed to Rosa26-lacZ reporter mice to analyze cre-mediated recombination. Line 2M was used for further studies. (J:150475)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
