Dp(XTceal3-Plp1)1Gmh

Micer clones MHPN312j24 (NCBIM37, 133123007-133127471) and MHPP264b06 (NCBIM37, 133374577-133383807) and cre mediated recombination were used to generate a tandem head-to-tail duplication of a 260.8 kb region between the two sites and a complete HPRT gene at the junction. The duplication spans from Tceal3 through 9.5 kb distal of Plp1. Presence of a duplication was confirmed in mice by semiquantitative multiplex PCR, Southern blot analysis, and FISH. qRT-PCR indicates an increase in Plp1, Tceal1, Tceal3, Morf4l2 and BC065397 transcript levels at multiple postnatal time points. (J:199809)