A floxed neo cassette was inserted upstream of exon 2 and a loxP site was inserted downstream of exon 3. Transient cre expression in ES cells removed the neo cassette leaving exons 2 and 3 floxed. (J:150148)
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A floxed neo cassette was inserted upstream of exon 2 and a loxP site was inserted downstream of exon 3. Transient cre expression in ES cells removed the neo cassette leaving exons 2 and 3 floxed. (J:150148)