Log In|Sign Up
EN
A targeting vector was constructed where an upstream loxP site was cloned into a blunted NsiI site in intron 7 and a floxed neomycin resistance cassette was cloned into a BglII site in intron 9. Targeting took place in PC3 ES cells homozygous for the cre transgene Tg(Prm-cre)70Og, which expresses Cre recombinase in male germ cells. Cre mediated recombination subsequently removed exons 8 and 9 upon transmission from male chimeric mice. Offspring from these male chimeras were selected for further breeding. This is considered a null allele. (J:149811)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
Show/Hide columns
| Phenotypes |
|---|
References Literature
Title
PMID
Journal
Year
IF
