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A BAC clone containing the Nkx2-5 locus was modified by homologous recombination in E. coli. A targeting vector containing the hrGFP reporter, FRT-flanked kanamycin/TK selection cassette, and Nkx2-5 3' and 5' homology arms was used to replace exon 1 and intron 1 of the Nkx2-5 locus via recombination in red-alpha and red-beta recombinase expressing cells. The selection cassette was excised from recombinant clones by induction of lambda PR-Flp in the bacterial cells. The recombined BAC DNA was microinjected into C57 mice. Four lines of transgenic mice were generated. (J:83506)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
