An IRES-lacZ/neo cassette replaced most of exon 2, and all of exons 3-5 as well as the intervening intronic sequences. Gene inactivation was confirmed by a lack of signal in immunoblot analysis of cortical neuron lysates. (J:148903)
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An IRES-lacZ/neo cassette replaced most of exon 2, and all of exons 3-5 as well as the intervening intronic sequences. Gene inactivation was confirmed by a lack of signal in immunoblot analysis of cortical neuron lysates. (J:148903)