A loxP/FRT flanked neomycin selection cassette replaced 6.6 kb of the locus, including exon 1 that contains the ATG start codon. Gene inactivation was confirmed by a lack of signal in RT-PCR analysis of ear skin. (J:148726)
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A loxP/FRT flanked neomycin selection cassette replaced 6.6 kb of the locus, including exon 1 that contains the ATG start codon. Gene inactivation was confirmed by a lack of signal in RT-PCR analysis of ear skin. (J:148726)