A frt- and neo-flanked neo cassette was inserted upstream of exon 1, and an additional loxP site was inserted downstream of exon 4. Germ line, flp-mediated recombination removed the neo cassette leaving exons 1 through 4 floxed. (J:148508)
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A frt- and neo-flanked neo cassette was inserted upstream of exon 1, and an additional loxP site was inserted downstream of exon 4. Germ line, flp-mediated recombination removed the neo cassette leaving exons 1 through 4 floxed. (J:148508)