Cpn1^tm1Raw

Exon 3 of the locus was replaced by a neomycin resistance cassette. RT-PCR analysis on homozygous liver extracts detected the presence of a truncated mRNA message corresponding to a splice variant that fused exon 2 to exon 4. Because exon 3 encoded a substrate binding site, it was hypothesized that any mutant protein transcribed from the truncated mRNA would lack carboxypeptidase activity. This was confirmed by a lack of terminal arginine cleavage on C3a and C5a complement proteins. (J:148315)