A loxP site was inserted upstream of exon 3, and an frt flanked neo cassette with a 3' loxP site. Germ line, flp mediated recombination was used to remove the neo cassette leaving exon 3 floxed. (J:148166)
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A loxP site was inserted upstream of exon 3, and an frt flanked neo cassette with a 3' loxP site. Germ line, flp mediated recombination was used to remove the neo cassette leaving exon 3 floxed. (J:148166)