A floxed neo cassette was inserted into intron 7, 720 bp downstream of exon 7, and a third loxP site was inserted upstream of exon 7 via homologous recombination. The neo cassette was then removed via Cre mediated recombination. (J:147275)
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A floxed neo cassette was inserted into intron 7, 720 bp downstream of exon 7, and a third loxP site was inserted upstream of exon 7 via homologous recombination. The neo cassette was then removed via Cre mediated recombination. (J:147275)