The gene trap vector, which contains a beta-geo cassette, inserted downstream of exon 5. The fusion product lacks the terminal 46 animo acids required to form the active heterodimer. The absence of endogenous protein expression but expression of the lacZ fusion transcript was confirmed by western blot analysis on mouse embryonic fibroblasts and a variety of tissues, respectively. (J:146618)
Basic Information
Insertion of gene trap vector
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count