A loxP site was inserted upstream of exon 5, and an frt flanked neo cassette with a 3' loxP site was inserted downstream of eoxn 5. Germ line, flp mediated recombination was used to remove the neo cassette leaving exon 5 floxed. (J:146538)
查看原文 参与反馈
A loxP site was inserted upstream of exon 5, and an frt flanked neo cassette with a 3' loxP site was inserted downstream of eoxn 5. Germ line, flp mediated recombination was used to remove the neo cassette leaving exon 5 floxed. (J:146538)