A loxP site was inserted upstream of exon 3, and an frt flanked neo cassette with a 5' loxP site was inserted downstream of exon 3. Germ line, flp mediated recombination was used to remove the neo cassette leaving exon 3 floxed. (J:146645)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count