A loxP site was inserted upstream of exon 2 and a FRT-flanked neomycin resistance cassette with a downstream loxP site was inserted into intron 6. Correct targeting was confirmed by genomic PCR. (J:146583)
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A loxP site was inserted upstream of exon 2 and a FRT-flanked neomycin resistance cassette with a downstream loxP site was inserted into intron 6. Correct targeting was confirmed by genomic PCR. (J:146583)